Photo of Dr. Lee Machado

Dr. Lee Machado

  • Job title: Senior Lecturer in Biochemistry
  • Department: Sports, Exercise & Life Sciences

General information

Lee is a Senior Lecturer in Biochemistry in the School of Health, co-leader of the Ageing Research Centre (ARC) in the Institute of Health and Wellbeing at the University of Northampton and Honorary Fellow in the Department of Genetics at the University of Leicester.

Lee completed an undergraduate degree at the University of Warwick and a PhD in cancer studies at the University of Birmingham. He conducted postdoctoral research at the Institute for Cancer Studies (Birmingham), the MRC toxicology unit (Leicester) and the Department of Genetics (Leicester) before working as a senior scientist for the cancer vaccine company, Scancell (Nottingham); Lee came to the University of Northampton as a Lecturer in 2013.

Teaching

Lee teaches on the following BSc Human Bioscience and Biology programmes:

  • SLS1008 Research Skills (Module Co-lead)
  • SLS2004 Processes of Life: Biochemistry (Module lead)
  • SLS3003 Immunology and Health (Module Lead)
  • SLS2010 (Teach)

Research

Lee’s research is focused on employing cellular and molecular genetic strategies to address how the host immune system responds to pathogens and cancer. The aim of this work is to increase our understanding of human health and disease and develop rational therapeutic approaches to harness the exquisite specificity and sensitivity of the immune system.

Key areas of research are:

  • Copy number variation of immunity genes
  • Tumour immunology and the development of cancer immunotherapy

Lee collaborates locally, nationally and internationally to advance his research agenda and has worked on Cancer Research UK, Medical Research Council and Wellcome Trust funded projects. He is a regular reviewer for a number of academic journals including Human Immunology, Blood, PLOSone and PLOS Genetics. He has published in high impact journals including Blood, American Journal of Human Genetics and the American Journal of Pathology and is an occasional reviewer for major funding bodies.

Current PhD Students:

  • Nermin Albalbeisi (The role of PAD2 in Ovarian cancer)
  • Amber Hameed (Characterisation of the glycome biosynthesis gene clusters involved in lipooligosaccharide and capsular polysaccharide biosynthesis in Campylobacter sp.)
  • Rafah Al-Zubaidi (The role of the tumour microenvironment in melanoma cell growth and vaccine induced anti-tumour responses in vivo)
  • Izzat Al-Rayahi (Role of complement protein Properdin in tumour growth and Metastasis)

Publications

Jump to: 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2003

2018

  1. Patel, P. M., Ottensmeier, C., Mulatero, C., Lorigan, P., Plummer, R., Pandha, H., Elsheik, S., Hadjimichael, E., Villasanti, N., Adams, S. E., Cunnell, M., Metheringham, R. L., Brentville, V. A., Machado, L., Daniels, I., Gijon, M., Hannaman, D. and Durrant, L. G. (2018) Targeting gp100 and TRP-2 with a DNA vaccine: incorporating T cell epitopes with a human IgG1 antibody induces potent T cell responses that are associated with favourable clinical outcome in a phase I/II trial. OncoImmunology. 2162-4011.

2017

  1. Poole, H., Sixsmith, J., Parkes, J., Ward, A., Pyer, M., Campbell, J., Machado, L., Rehling, T., Youell, J., Carter, J., Oyebode, J., O’Malley, M., Anthony, K., Rogers, S., Jones, J., Siddons, L., Smith, L.-A., Kay, T., Fraser, M., Score, J., El Khoury, L., Hameed, A., Albalbeisi, N., Durrant, L. G., Al-Zubaidi, R., Al-Rayahi, I., da Silva, K. and Taylor, J. (2017) Health and wellbeing amongst older people research in Northamptonshire. East Midlands Research into Ageing Network (EMRAN) Discussion Paper Series. 15, pp. 1-33. 2059-3341.
  2. Machado, L., Moseley, P. M., Moss, R., Deen, S., Nolan, C., Spendlove, I. and Ramage, J. (2017) High mobility group protein B1 is a predictor of poor survival in ovarian cancer. Oncotarget. 8(60), pp. 101215-101223. 1949-2553.
  3. Zuccherato, L., Schneider, S., Tarazona-Santos, E., Hardwick, R. J., Berg, D., Bogle, H., Gouveia, M., Machado, L., Machado, M., Rodrigues-Soares, F., Soares-Souza, G., Togni, D., Zamudio, R., Gilman, R., Duarte, D., Hollox, E. J. and Rodrigues, M. R. (2017) Population genetics of immune-related multilocus CNV in Native Americans. Journal of the Royal Society Interface. 14(128) 1742-5689.

2016

  1. Machado, L., Moseley, P. M., Moss, R., Deen, S., Nolan, C., Spendlove, I., Ramage, J., Chan, S. and Durrant, L. G. (2016) Downregulation of the citrullinating enzyme Peptidyl-arginine deiminase type-2 in ovarian cancer indicates poor prognosis. Poster presented to: National Cancer Research Institute (NCRI) Cancer Conference, Liverpool, UK, 06-09 November 2016.
  2. Rahbari, R., Zuccherato, L., Tischler, G., Chihota, B., Tarazona-Santos, E., Machado, L. and Hollox, E. J. (2016) Understanding the genomic structure of copy number variants of the low-affinity Fcγ receptor region allows confirmation of the association of FCGR3B deletion with rheumatoid arthritis. Poster presented to: British Society for Immunology/Dutch Society for Immunology (BSI/NVVI) Annual Congress 2016, Liverpool, 06-09 December 2016.
  3. Rahbari, R., Zuccherato, L. W., Tischler, G., Chihota, B., Ozturk, H., Saleem, S., Tarazona-Santos, E., Machado, L. and Hollox, E. J. (2016) Understanding the genomic structure of copy number variants of the low-affinity Fcγ receptor region allows confirmation of the association of FCGR3B deletion with rheumatoid arthritis. Human Mutation. 1059-7794.

2015

  1. Durrant, L. G., Ottensmeier, C. H., Mulatero, C., Lorigan, P., Plummer , R., Cunnell, M., Metheringham, R., Brentville, V., Machado, L., Daniels , I., Hannaman, D. and Patel, P. M. (2015) A clinical trial of a DNA vaccine (SCIB1) that targets dendritic cells in vivo in fully resected melanoma patients; a vaccine to prevent disease recurrence? Poster presented to: 15th International Conference on Progress in Vaccination Against Cancer (PIVAC-15), Tübingen, Germany, 06-08 October 2015.
  2. Haridan, U. S., Mokhtar, U., Machado, L., Abdul Aziz, A. T., Shueb, R. H., Zaid, M., Sim, B., Mustafa, M., Nik Yusof, N. K., Lee, C. K. C., Abu Bakar, S., AbuBakar, S., Hollox, E. J. and Boon Peng, H. (2015) A comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PLoS ONE. 10(1) 1932-6203.
  3. Patel, P. M., Ottensmeier, C., Mulatero, C., Lorigan, P., Plummer, R., Hannaman, D., Cunnell, M., Metheringham, R. L., Brentville, V. A., Daniels, I., Machado, L. and Durrant, L. G. (2015) Abstract 9035: An adjuvant clinical trial of SCIB1, a DNA vaccine that targets dendritic cells in vivo, in fully resected melanoma patients. Journal of Clinical Oncology. 33(15 Sup) 0732-183X.
  4. Machado, L. and Ottolini, B. (2015) An evolutionary history of defensins: a role for copy number variation in maximizing host innate and adaptive immune responses. Frontiers in Immunology. 6(115) 1664-3224.
  5. Hargreaves, C. E., Iriyama, C., Rose-Zerilli, M. J. J., Nagelkerke, S. Q., Hussain, K., Ganderton , R., Lee, C., Machado, L., Hollox, E. J., Parker, H., Latham, K. V., Kuijpers, T. W., Potter, K. N., Coupland, S. E., Davies, A., Stackpole, M., Oates, M., Pettitt, A. R., Glennie, M. J., Cragg, M. S. and Strefford, J. C. (2015) Evaluation of high-throughput genomic assays for the Fc gamma receptor locus. PLoS ONE. 10(11) 1932-6203.
  6. Hargreaves, C. E., Rose-Zerilli, M. J. J., Machado, L., Iriyama, C., Hollox, E. J., Cragg, M. S. and Strefford, J. C. (2015) Fcγ receptors: genetic variation, function, and disease. Immunological Reviews. 268(1), pp. 6-24. 1600-065X.
  7. Zheng, Y., Parsonage, G., Zhuang, X., Machado, L., James, C., Salman, A., Searle, P., Hui, E. P., Chan, A. T. C. and Lee, S. P. (2015) Human Leukocyte Antigen (HLA) A*1101-restricted Epstein-Barr virus-specific T-cell receptor gene transfer to target Nasopharyngeal carcinoma. Cancer Immunology Research. 3(10), pp. 1138-1147. 2326-6066.

2014

  1. Durrant, L. G., Ottensmeier, C., Lorigan, P., Mulatero, C., Plummer, R., Cunell, M., Metheringham, R. L., Brentville, V. A. and Machado, L. (2014) Abstract CT331: Phase I/II trial of a novel antibody DNA immunotherapy, targeting CD64, in the treatment of Melanoma. Cancer Research. 74(19 Sup) 0008-5472.
  2. Durrant, L. G., Xue, L., Machado, L., Metheringham, R. L. and Brentville, V. A. (2014) DNA vaccines. In: Rees, R. C. (ed.) Tumor Immunology and Immunotherapy. Oxford: Oxford University Press. pp. 251-264.
  3. Hargreaves, C., Iriyama, C., Rose-Zerilli, M., Lee, C., Potter, K., Ganderton, R., Hussain, K., Machado, L., Hollox, E. J., Coupland, S., Stackpole, M., Oates, M., Pettitt, A. R., Cragg, M. and Strefford, J. (2014) Genomic dissection of the Fcγ receptor region in the context of monoclonal antibody therapy. Blood. 124(21), p. 2996. 0006-4971.
  4. Machado, L., Moseley, P., Moss, R., Nolan, C., Rampage, J., Chan, S. and Durrant, L. G. (2014) High-mobility group protein 1 (HMGB1) is an independent predictor of poor survival in ovarian cancer. Poster presented to: National Cancer Research Institute (NCRI) Cancer Conference, Liverpool, UK, 02-05 November 2014. Also presented at: British Society for Immunology Annual Congress (BSI2014), Brighton, UK, 01-04 December 2014 (Unpublished)
  5. Patel, P. M., Durrant, L. G., Ottensmeier, C., Mulatero, C., Lorigan, P., Plummer, R., Cunnell, M., Metheringham, R. L., Brentville, V. A., Machado, L., Daniels, I. and Hannaman, D. (2014) Phase I trial of ImmunoBody in melanoma patients. Poster presented to: 2014 American Society of Clinical Oncology (ASCO) Annual Meeting, McCormick Place, Chicago, Illinois, 30 May - 03 June 2014. (Unpublished)
  6. Dupont, A., Mohamed, F., Salehen, N.'A., Glenn, S., Francescut, L., Adib, R., Byrne, S., Brewin, H., Elliott, I., Richards, L., Dimitrova, P., Schwaeble, W., Ivanovska, N., Kadioglu, A., Machado, L., Andrew, P. W. and Stover, C. (2014) Septicaemia models using Streptococcus pneumoniae and Listeria monocytogenes: understanding the role of complement properdin. Medical Microbiology and Immunology. 203(4), pp. 257-271. 0300-8584.

2013

  1. Machado, L., Bowdrey, J., Ngaimisi, E., Habtewold, A., Minzi, O., Makonnen, E., Yimer, G., Amogne, W., Mugusi, S., Janabi, M., Aderaye, G., Mugusi, F., Viskaduraki, M., Aklillu, E. and Hollox, E. J. (2013) Copy number variation of Fc gamma receptor genes in HIV-infected and HIV-tuberculosis co-infected individuals in Sub-Saharan Africa. PLoS ONE. 8(11), pp. 1-8. 1932-6203.

2012

  1. Hardwick, R. J., Amogne, W., Mugusi, S., Yimer, G., Ngaimisi, E., Habtewold, A., Minzi, O., Makonnen, E., Janabi, M., Machado, L., Viskaduraki, M., Mugusi, F., Aderaye, G., Lindquist, L., Hollox, E. J. and Aklillu, E. (2012) B-defensin genomic copy number is associated with HIV load and immune reconstitution in sub-Saharan Africans. The Journal of Infectious Diseases. 206(7), pp. 1012-1019. 1537-6613.
  2. Parsonage, G., Machado, L., Hui, J. W.-Y., McLarnon, A., Schmaler, T., Balasothy, M., To, K.-F., Vlantis, A. C., Hasselt, C. A. V., Lo, K.-W., Wong, W.-L., Hui, E. P., Cheung Chan, A. T. and Lee, S. P. (2012) CXCR6 and CCR5 localize T lymphocyte subsets in nasopharyngeal carcinoma. American Journal of Pathology. 180(3), pp. 1215-1222. 0002-9440.
  3. Machado, L., Hardwick, R. J., Bowdrey, J., Bogle, H., Knowles, T., Sironi, M. and Hollox, E. J. (2012) Evolutionary history of copy-number-variable locus for the low-affinity Fcy receptor: mutation rate, autoimmune disease, and the legacy of helminth infection. The American Journal of Human Genetics. 90(6), pp. 973-985. 0002-9297.

2011

  1. Hardwick, R. J., Machado, L., Zuccherato, L., Antolinos, S., Xue, Y., Shawa, N., Gilman, R., Cabrera, L., Berg, D., Tyler-Smith, C., Kelly, P., Tarazona-Santos, E. and Hollox, E. J. (2011) A worldwide analysis of beta-defensin copy number variation suggests recent selection of a high-expressing DEFB103 gene copy in East Asia. Human Mutation. 32(7), pp. 743-750. 1059-7794.
  2. Machado, L., Hardwick, R. J., Bogle, H., Bowdrey, J., Sironi, M. and Hollox, E. J. (2011) Evolutionary history of the low-affinity Fc gamma receptor copy number variable locus: diversity, disease and helminth infection. Poster presented to: Annual Congress of the British Society for Immunology, Liverpool, UK, 05-08 December 2011. (Unpublished)
  3. Hardwick, R. J., Amogne, W., Mugusi, S., Yimer, G., Ngaimisi, E., Habtewold, A., Minzi, O., Makonnen, E., Janabi, M., Machado, L., Mugusi, F., Aderaye, G., Lindquist, L., Hollox, E. J. and Aklillu, E. (2011) β-Defensin genomic copy number is associated with HIV viral load and immune reconstitution in sub-Saharan Africans. Poster presented to: Annual Congress of the British Society for Immunology, Liverpool, UK, 05-08 December 2011. (Unpublished)

2010

  1. Zheng, Y., Machado, L., Johnson, B., James, C., Parsonage, G. and Lee, S. P. (2010) EBV-specific T cell receptor gene transfer to target nasopharyngeal carcinoma. Poster presented to: 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases, University of Birmingham, 04-07 September 2010. (Unpublished)
  2. Parsonage, G., Machado, L., Hui, J. W.-Y., Schmaler, T. T., Balasothy, M., Van Hasselt, A., Vlantis, L., Hui, E. P., Chan, A. T. C., Lo, K.-W. and Lee, S. P. (2010) T cell homing to nasopharyngeal carcinoma. Poster presented to: 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases, University of Birmingham, 04-07 September 2010. (Unpublished)

2009

  1. Russell, L., Capasso, M., Vater, I., Akasaka, T., Bernard, O., Calasanz, M. J., Chandrasekaran, T., Chapiro, E., Gesk, S., Griffiths, M., Guttery, D., Haferlach, C., Harder, L., Heidenreich, O., Irving, J., Kearney, L., Nguyen-Khac, F., Machado, L., Minto, L., Majid, A., Moorman, A., Morrison, H., Rand, V., Strefford, J., Schwab, C., Tonnies, H., Dyer, M., Siebert, R. and Harrison, C. (2009) Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. Blood. 114(13), pp. 2688-2698. 0006-4971.
  2. Machado, L., Jarrett, R., Morgan, S., Murray, P., Hunter, B., Hamilton, E., Crocker, J., Thomas, W., Steven, N., Ismail, T., Chapman, A., Adams, D. H. and Lee, S. P. (2009) Expression and function of T cell homing molecules in Hodkins's lymphoma. Cancer Immunology, Immunotherapy. 58(1), pp. 85-94. 0340-7004.
  3. Zheng, Y., Machado, L., Johnson, B., James, C., Parsonage, G. and Lee, S. P. (2009) T cell receptor gene transfer to target the Epstein-Barr virus-associated malignancy nasopharyngeal carcinoma. Poster presented to: National Cancer Research Institute (NCRI) Cancer Conference, University of Birmingham, UK, 05-07 October 2009. (Unpublished)

2008

  1. Baumforth, K. R. N., Birgersdotter, A., Reynolds, G. M., Wei, W., Kapatai, G., Flavell, J., Kalk, E., Piper, K., Lee, S. P., Machado, L., Hadley, K., Sundblad, A., Sjoberg, J., Bjorkholm, M., Porwit, A., Yap, L.-F., Teo, S., Grundy, R., Young, L. S., Ernberg, I., Woodman, C. and Murray, P. (2008) Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's Lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells. The American Journal of Pathology. 173(1), pp. 195-204. 0002-9440.
  2. Russell, L., Capasso, M., Vater, I., Moorman, A., Akasaka, T., Harder, L., Heidenreich, O., Machado, L., Gest, S., Strefford, J., Chandrasekaran, T., Tonnies, H., Majid, A., Bernard, O., Dyer, M., Siebert, R. and Harrison, C. (2008) IGH@ translocations involving the pseudoautosomal region 1 (PAR1) of both sex chromosomes deregulate the cytokine receptor-like factor 2 (CRLF2) gene in B cell precursor acute lymphoblastic leukemia (BCP-ALL). Paper presented to: 50th American Society of Hematology (ASH) Annual Meeting and Exposition, San Francisco, California, 06-09 December 2008. (Unpublished)
  3. Palendira, U., Chinn, R., Raza, W., Piper, K., Pratt, G., Machado, L., Bell, A., Khan, N., Hislop, A., Steyn, R., Rickinson, A., Buckley, C. and Moss, P. (2008) Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow. Blood. 112(8), pp. 3293-3302. 0006-4971.

2007

  1. Machado, L., Schmaler, T. T., Balasothy, M., Morgan, S., Larsen, M., Hislop, A., Murray, P., Mack, M., Adams, D., Van Hasselt, A., Hui, E. P., Chan, A. T. C. and Lee, S. P. (2007) CXCR6 and CCR5 mediate recruitment of CD4 and CD8 T lymphocytes to nasopharyngeal carcinoma. Poster presented to: Cancer Research UK Midlands Regional Meeting, Birmingham, UK, April 2007. (Unpublished)
  2. Machado, L., Jarrett, R., Morgan, S., Murray, P., Hunter, B., Crocker, J., Thomas, W., Steven, N., Ismail, T., Chapman, A., Adams, D. and Lee, S. P. (2007) Molecular mechanisms for T cell recruitment to Hodgkin's lymphoma. Poster presented to: Keystone Symposia on Molecular and Cellular Biology: The Potent New Anti-Tumor Immunotherapies, Banff, Alberta, Canada, 28 March - 02 April 2007. (Unpublished)

2006

  1. Hamilton, E., Machado, L., Reynolds, G. M., Taniere, P., Lee, S. P., Steven, N. and Ismail, T. (2006) Biomarkers of the immune response in primary colorectal cancer. Poster presented to: Annual Meeting of the British Society of Gastroenterology, Surgical Section in Association with the Association of Coloproctology (ACPGBI) and IBD Section Joint Symposium, Birmingham, UK, 20-23 March 2006. (Unpublished)

2005

  1. Lee, S. P., Green, A. M., Machado, L., Patel, P., Thomas, W., Goodyear, O., Briggs, D., McDonald, D., Marsden, J. and Steven, N. (2005) Infusing advanced cancer patients with tumour antigen-specific CD4+ and CD8+ T cell clones. Poster presented to: Annual Congress of the British Society for Immunology, Harrogate, UK, 06-09 December 2005. (Unpublished)

2003

  1. Lee, S. P., Hewitt, A., Thomas, W., Machado, L., Goodyear, O., Marsden, J., Van der, B., Briggs, D. and Steven, N. (2003) Generating cancer/testis antigen- and Epstein-Barr virus-specific T cell clones for adoptive immunotherapy. Poster presented to: Keystone Symposia on Molecular and Cellular Biology: Basic Aspects of Tumour Immunology, Colorado, USA, 2003. (Unpublished)
This list was generated from NECTAR on Sun May 20 02:57:00 2018 BST.

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